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stemspan megakaryocyte expansion supplement  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc stemspan megakaryocyte expansion supplement
    Stemspan Megakaryocyte Expansion Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemspan+megakaryocyte+expansion+supplement/pm39354676-150-16-20?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    stemspan megakaryocyte expansion supplement - by Bioz Stars, 2026-06
    90/100 stars

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    stemspan megakaryocyte expansion supplement  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc stemspan megakaryocyte expansion supplement
    Stemspan Megakaryocyte Expansion Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemspan+megakaryocyte+expansion+supplement/pm39354676-150-16-20?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    stemspan megakaryocyte expansion supplement - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier
    stemspan™ megakaryocyte expansion supplement  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc stemspan™ megakaryocyte expansion supplement
    Expansion and maturation of hiPSC-derived megakaryocytes (MKs) in different media conditions. ( A ) Experimental outline using the improved induction conditions. Megakaryocyte progenitors (MKPs) derived from stage II at days 9, 12, and 14 (purple arrows) were harvested and matured into MKs in stage III for up to 12 additional days in three different media <t>(StemSpan,</t> IMDM or APEL), supplemented as indicated. From day 9 + 10 on, MKs were cultured under dynamic conditions at 60 rpm. Cells were harvested, counted, and analyzed by flow cytometry (platelet counts are shown in B,C). ( B ) The total amount of CD61 + /CD41a + /CD42b + maturing MKs revealed no significant difference after culture in the three different media (StemSpan, down pointing triangle; IMDM, squares; APEL, upward pointing triangles; three different mean value lines representing the three media; symbol color depicts the two independent hiPSC clones UCB144-CT2-C, grey and BIHi001-A, black; n = 4, p > 0.05; two-way ANOVA, multiple comparisons). ( C ) MKs showed various maturation stages, indicated by two or more nuclei (deep purple), independent of media conditions (May–Grünwald Giemsa stain, MGG; top row, lower magnification inserts). Characteristic MK immunophenotype CD42b (green), CD61 (red); nuclear DAPI stain (blue; lower row). Representative pictures of two replicates of clone BIHi001-A are shown after harvest on day 9 + 7 (stage III) and stained as specified.
    Stemspan™ Megakaryocyte Expansion Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stemspan+megakaryocyte+expansion+supplement/pmc08348107-174-24-28?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    stemspan™ megakaryocyte expansion supplement - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Expansion and maturation of hiPSC-derived megakaryocytes (MKs) in different media conditions. ( A ) Experimental outline using the improved induction conditions. Megakaryocyte progenitors (MKPs) derived from stage II at days 9, 12, and 14 (purple arrows) were harvested and matured into MKs in stage III for up to 12 additional days in three different media (StemSpan, IMDM or APEL), supplemented as indicated. From day 9 + 10 on, MKs were cultured under dynamic conditions at 60 rpm. Cells were harvested, counted, and analyzed by flow cytometry (platelet counts are shown in B,C). ( B ) The total amount of CD61 + /CD41a + /CD42b + maturing MKs revealed no significant difference after culture in the three different media (StemSpan, down pointing triangle; IMDM, squares; APEL, upward pointing triangles; three different mean value lines representing the three media; symbol color depicts the two independent hiPSC clones UCB144-CT2-C, grey and BIHi001-A, black; n = 4, p > 0.05; two-way ANOVA, multiple comparisons). ( C ) MKs showed various maturation stages, indicated by two or more nuclei (deep purple), independent of media conditions (May–Grünwald Giemsa stain, MGG; top row, lower magnification inserts). Characteristic MK immunophenotype CD42b (green), CD61 (red); nuclear DAPI stain (blue; lower row). Representative pictures of two replicates of clone BIHi001-A are shown after harvest on day 9 + 7 (stage III) and stained as specified.

    Journal: International Journal of Molecular Sciences

    Article Title: Improving Human Induced Pluripotent Stem Cell-Derived Megakaryocyte Differentiation and Platelet Production

    doi: 10.3390/ijms22158224

    Figure Lengend Snippet: Expansion and maturation of hiPSC-derived megakaryocytes (MKs) in different media conditions. ( A ) Experimental outline using the improved induction conditions. Megakaryocyte progenitors (MKPs) derived from stage II at days 9, 12, and 14 (purple arrows) were harvested and matured into MKs in stage III for up to 12 additional days in three different media (StemSpan, IMDM or APEL), supplemented as indicated. From day 9 + 10 on, MKs were cultured under dynamic conditions at 60 rpm. Cells were harvested, counted, and analyzed by flow cytometry (platelet counts are shown in B,C). ( B ) The total amount of CD61 + /CD41a + /CD42b + maturing MKs revealed no significant difference after culture in the three different media (StemSpan, down pointing triangle; IMDM, squares; APEL, upward pointing triangles; three different mean value lines representing the three media; symbol color depicts the two independent hiPSC clones UCB144-CT2-C, grey and BIHi001-A, black; n = 4, p > 0.05; two-way ANOVA, multiple comparisons). ( C ) MKs showed various maturation stages, indicated by two or more nuclei (deep purple), independent of media conditions (May–Grünwald Giemsa stain, MGG; top row, lower magnification inserts). Characteristic MK immunophenotype CD42b (green), CD61 (red); nuclear DAPI stain (blue; lower row). Representative pictures of two replicates of clone BIHi001-A are shown after harvest on day 9 + 7 (stage III) and stained as specified.

    Article Snippet: Where indicated, final maturation was performed with StemSpan TM animal component free (ACF) medium (Stemcell Technologies) with 5 IU/mL heparin (Merck Millipore) and 1× StemSpan™ megakaryocyte expansion supplement (Stemcell Technologies) as described [ ], or StemDiff TM APEL2 (Stemcell Technologies) containing 5% PFHM-II (Thermo Fisher Scientific), SCF (50 ng/mL, PeproTech) and TPO (50 ng/mL, Stemcell Technologies) as described [ ].

    Techniques: Derivative Assay, Cell Culture, Flow Cytometry, Clone Assay, Giemsa Stain, Staining

    Human iPSC-derived megakaryocytes (MKs) produce pro-platelets and release platelets in all media conditions. ( A ) MKs with pro-platelet structures on day 9 + 12 (stage III) after dynamic culture in supplemented StemSpan, IMDM, and APEL media. Representative immunofluorescence images of MKs from one hiPSC clone (UCB144-CT2-C) stained with CD42b (green), CD61 (red), and DAPI (nuclei, blue) are shown. Pro-platelets are indicated by white arrows. ( B ) Platelet counts on day 9 + 12 (stage III) calculated per input MK were significantly increased after culture in supplemented IMDM medium, compared to StemSpan ( p = 0.046) and APEL medium ( p = 0.0273; both unpaired t-tests * p < 0.05 and ** p < 0.01). ( C ) Additional analysis showed comparable platelet counts per 1 × 10 6 input hiPSCs ( p > 0.05) in all media conditions. Results from two independent hiPSC clones (UCB144-CT2-C, grey circles, and BIHi001-A, black diamonds; n = 8, measured in duplicates).

    Journal: International Journal of Molecular Sciences

    Article Title: Improving Human Induced Pluripotent Stem Cell-Derived Megakaryocyte Differentiation and Platelet Production

    doi: 10.3390/ijms22158224

    Figure Lengend Snippet: Human iPSC-derived megakaryocytes (MKs) produce pro-platelets and release platelets in all media conditions. ( A ) MKs with pro-platelet structures on day 9 + 12 (stage III) after dynamic culture in supplemented StemSpan, IMDM, and APEL media. Representative immunofluorescence images of MKs from one hiPSC clone (UCB144-CT2-C) stained with CD42b (green), CD61 (red), and DAPI (nuclei, blue) are shown. Pro-platelets are indicated by white arrows. ( B ) Platelet counts on day 9 + 12 (stage III) calculated per input MK were significantly increased after culture in supplemented IMDM medium, compared to StemSpan ( p = 0.046) and APEL medium ( p = 0.0273; both unpaired t-tests * p < 0.05 and ** p < 0.01). ( C ) Additional analysis showed comparable platelet counts per 1 × 10 6 input hiPSCs ( p > 0.05) in all media conditions. Results from two independent hiPSC clones (UCB144-CT2-C, grey circles, and BIHi001-A, black diamonds; n = 8, measured in duplicates).

    Article Snippet: Where indicated, final maturation was performed with StemSpan TM animal component free (ACF) medium (Stemcell Technologies) with 5 IU/mL heparin (Merck Millipore) and 1× StemSpan™ megakaryocyte expansion supplement (Stemcell Technologies) as described [ ], or StemDiff TM APEL2 (Stemcell Technologies) containing 5% PFHM-II (Thermo Fisher Scientific), SCF (50 ng/mL, PeproTech) and TPO (50 ng/mL, Stemcell Technologies) as described [ ].

    Techniques: Derivative Assay, Immunofluorescence, Staining, Clone Assay

    Media conditions.

    Journal: International Journal of Molecular Sciences

    Article Title: Improving Human Induced Pluripotent Stem Cell-Derived Megakaryocyte Differentiation and Platelet Production

    doi: 10.3390/ijms22158224

    Figure Lengend Snippet: Media conditions.

    Article Snippet: Where indicated, final maturation was performed with StemSpan TM animal component free (ACF) medium (Stemcell Technologies) with 5 IU/mL heparin (Merck Millipore) and 1× StemSpan™ megakaryocyte expansion supplement (Stemcell Technologies) as described [ ], or StemDiff TM APEL2 (Stemcell Technologies) containing 5% PFHM-II (Thermo Fisher Scientific), SCF (50 ng/mL, PeproTech) and TPO (50 ng/mL, Stemcell Technologies) as described [ ].

    Techniques: